Figure 4.

Differentiation of dendritic cells derived from wild-type and HLA DR knockout iPSCs. (a) Schematic representation of differentiation protocol for iPSCs into dendritic cells. (b) iPS cell-derived dendritic cells on day 4 in the 1^st step, day 6 in the 2^nd step, day 13 in the 3^rd step, day 24 in the 4^th step, and day 31 in the 5^th step were analyzed for the expression of brachyury, CD34, CD45, CD14, and CD83, respectively. (c) May-Grunwald-Giemsa staining of mature iPS induced DC on the glass slide is shown.