Figure 2.

Effect of HSYA on HR-induced myocardial apoptosis in NPCMs. NPCMs were preincubated with HSYA (2.5, 5, and 10 μM) and nisoldipine (100 nM) for 24 h, followed by hypoxia (6 h) and reoxygenation (24 h). (a) Representative images of TUNEL staining (scale bar, 200 μm). (b) Representative images of JC-1 staining (scale bar, 200 μm). (c) Analysis of TUNEL staining results. (d) Analysis of JC-1 staining results. Data are expressed as the mean ± SD. ^###P < 0.001 vs. control group; ^∗P < 0.05 vs. HR group; ^∗∗∗P < 0.001 vs. HR group.