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. 2021 Feb 24;320(5):C731–C741. doi: 10.1152/ajpcell.00425.2020

Figure 2.

Figure 2.

Down-regulation of p110α or p110δ reduces lysoPC-induced PIP3 production. A: Bovine aortic ECs were transiently transfected with NsiRNA (40 nmol/L), p110α siRNA (20 nmol/L), or p110δ siRNA (20 nmol/L). PIP3 production in the presence or absence of lysoPC (12.5 μmol/L) was determined by ELISA and represented by scatter plot (n = 3 independent biological samples, a. P < 0.001 compared with medium, b. P < 0.001 compared with NsiRNA, c. P < 0.001 compared with p110α siRNA, d. P < 0.001 compared with p110δ siRNA, e. P<0.001 compared with NsiRNA + lysoPC). B: Bovine aortic ECs were transiently transfected with NsiRNA (40 nmol/L), p110β siRNA (30 nmol/L), or p110γ siRNA (30 nmol/L). PIP3 production in the presence or absence of lysoPC (12.5 μmol/L) was determined by ELISA and represented by scatter plot (n = 3 independent biological samples, a. P < 0.001 compared with medium, b. P < 0.001 compared with NsiRNA, c. P < 0.001 compared with p110β siRNA, d. P < 0.001 compared with p110γ siRNA). Statistical analysis for A, B performed using One-Way ANOVA with Tukey’s Multiple Comparisons Test.