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. 2021 Feb 24;320(5):C731–C741. doi: 10.1152/ajpcell.00425.2020

Figure 5.

Figure 5.

Down-regulation of p110α and p110δ partially preserves EC migration in presence of lysoPC. Bovine aortic ECs were transiently transfected for 24 h with NsiRNA (40 nmol/L), p110α siRNA (20 nmol/L), or p110δ siRNA (20 nmol/L) and then made quiescent for 12 h. The migration assay was initiated and migration in the presence or absence of lysoPC (12.5 μmol/L) was assessed after 24 h. (Upper) Representative images are shown at 40x magnification (Scale Bar, 200 µm). Arrow indicates the starting line of EC migration. (Lower) EC migration represented in scatter plots for (A) p110α down-regulation (n = 3 independent biological samples, a. P < 0.001 compared with medium, b. P < 0.001 compared with NsiRNA, c. P < 0.001 compared with p110α siRNA, and d. P < 0.001 compared with p110α siRNA + lysoPC) and (B) p110δ down-regulation (n = 3 independent biological samples, e. P < 0.001 compared with medium, f. P < 0.001 compared with NsiRNA, g. P < 0.001 compared with p110δ siRNA, and h. P < 0.001 compared with p110δ siRNA + lysoPC). Statistical analysis performed using One-Way ANOVA with Tukey’s Multiple Comparisons Test.