. 2021 Mar 5;320(5):H1822–H1835. doi: 10.1152/ajpheart.00673.2020

Table 1.

Percentage of phosphorylated peptides as observed by mass spectrometry after AP and subsequent PKA treatments

Phosphorylated Amino Acid	Phosphopeptide Observed	%Phosphorylation
Phosphorylated Amino Acid	Phosphopeptide Observed	AP	PKA
Ser-273	²⁷²TS_pLAGAGR²⁷⁹	55 ± 11	68 ± 11 (13)
	²⁷¹RTS_pLAGAGR²⁷⁹
Ser-282	²⁸¹TS_pDSHEDAGTLDFSSLLK²⁹⁸	84 ± 8	91 ± 5 (7)
	²⁸⁰RTS_pDSHEDAGTLDFSSLLK²⁹⁸
Ser-302	²⁹⁹KRDS_pFR³⁰⁴	56 ± 12	63 ± 12 (7)
Ser-307	³⁰⁵RDS_pKLEAPAEEDVWEILR³²²	3 ± 2	27 ± 6 (24)
Average		50 ± 2	62 ± 13 (12)

Values are means ± SD (%change in phosphorylation due to PKA treatment). Average refers to average phosphorylated percentage and associated SD calculated as square root of the sum of variances to reflect error propagation. AP, alkaline phosphatase; PKA, protein kinase A.