Table 2.
Results for maximum developed tension (Tdev) at pCa 4.8
| Tdev (kPa) | NTGβ | NTGβ,PKA | t/tβ | t/tβ,PKA | ANOVA |
|---|---|---|---|---|---|
| n | 9 | 8 | 10 | 10 | |
| Pi (mM) = 0 | 25.33 ± 1.60 | 14.98 ± 2.37 | 17.52 ± 1.76 | 13.38 ± 1.26 | Main effects |
| 1 | 21.49 ± 1.21 | 13.90 ± 2.41 | 16.34 ± 1.64 | 12.67 ± 1.11 | *MYBPC3, †Pi, †PKA |
| 2 | 20.34 ± 1.01 | 13.14 ± 2.23 | 15.10 ± 1.45 | 11.68 ± 1.03 | |
| 3 | 19.20 ± 1.03 | 11.98 ± 2.07 | 13.87 ± 1.27 | 10.66 ± 0.92 | Interactions |
| 4 | 18.38 ± 0.80 | 11.23 ± 1.89 | 13.05 ± 1.19 | 9.90 ± 0.91 | *Pi×PKA |
| 5 | 17.74 ± 0.72 | 10.53 ± 1.71 | 12.36 ± 1.20 | 9.09 ± 0.79 | |
| 8 | 16.37 ± 0.43 | 9.22 ± 1.46 | 10.72 ± 1.11 | 7.63 ± 0.72 | |
| 12 | 14.18 ± 0.73 | 7.88 ± 1.22 | 9.05 ± 1.01 | 6.02 ± 0.68 | |
| ΔTdev/ΔPi | −1.38 ± 0.17 | −0.90 ± 0.18 | −1.05 ± 0.16 | −0.88 ± 0.14 | *PKA |
Values are means ± SE; n, number of strips. Statistical results reflect repeated-measures ANOVA and demonstrate statistical significance for the main effects of MYBPC3, Pi, PKA, and for interactions Pi × MYBPC3, PKA × MYBPC3, Pi × PKA, Pi × PKA × MYBPC3. The sensitivity of Tdev to Pi was calculated by linear regression using values measured at 0–5 mM Pi and denoted here as ΔTdev/ΔPi. Two-way ANOVA was then used to determine whether ΔTdev/ΔPi was influenced by the absence of cMyBP-C and/or PKA. *P < 0.05, †P < 0.01. NTGβ, nontransgenic; t/tβ, transgenic lacking cMyBP-C; PKA, protein kinase A.