Table 6.
Results for rate of myosin detachment rate, 2πc, at maximal pCa 4.8 and submaximal pCa 5.75
| 2πc (s-1) at pCa 4.8 | NTGβ | NTGβ,PKA | t/tβ | t/tβ,PKA | ANOVA |
|---|---|---|---|---|---|
| n | 9 | 8 | 10 | 10 | |
| Pi (mM) = 0 | 15.75 ± 1.08 | 20.24 ± 1.44 | 20.63 ± 1.43 | 21.35 ± 1.66 | Main effects |
| 1 | 16.77 ± 1.56 | 21.29 ± 1.54 | 21.17 ± 1.01 | 24.36 ± 1.91 | *MYBPC3, †Pi, *PKA |
| 2 | 17.98 ± 1.69 | 22.07 ± 1.71 | 22.28 ± 0.92 | 26.08 ± 1.82 | |
| 3 | 19.17 ± 1.70 | 23.82 ± 1.76 | 23.33 ± 0.80 | 27.76 ± 1.84 | Interactions |
| 4 | 20.63 ± 1.74 | 24.55 ± 1.85 | 24.62 ± 0.77 | 28.80 ± 2.01 | †Pi × MYBPC3 |
| 5 | 21.98 ± 1.70 | 26.30 ± 1.90 | 26.39 ± 0.95 | 31.07 ± 1.96 | |
| 8 | 24.20 ± 1.78 | 29.02 ± 1.87 | 28.99 ± 1.19 | 34.25 ± 2.04 | |
| 12 | 27.07 ± 1.84 | 31.06 ± 1.81 | 32.16 ± 1.75 | 37.81 ± 1.95 | |
| Δ2πc/ΔPi | 1.15 ± 0.12 | 1.20 ± 0.18 | 1.32 ± 0.13 | 1.71 ± 0.17 | *MYBPC3 |
| 2πc (s-1) at pCa 5.75 | |||||
| Pi (mM) = 0 | 17.11 ± 1.34 | 19.56 ± 1.08 | 22.33 ± 1.55 | 24.99 ± 1.72 | Main effects |
| 1 | 19.01 ± 1.65 | 20.92 ± 1.12 | 23.22 ± 1.51 | 27.46 ± 2.09 | †MYBPC3, †Pi |
| 2 | 19.74 ± 1.64 | 22.62 ± 1.00 | 24.40 ± 1.49 | 29.60 ± 2.45 | |
| 3 | 22.14 ± 1.83 | 24.67 ± 1.19 | 25.84 ± 1.58 | 31.20 ± 2.68 | Interactions |
| 4 | 23.25 ± 1.86 | 26.10 ± 1.52 | 27.79 ± 1.81 | 33.03 ± 2.55 | †Pi × MYBPC3 |
| 5 | 24.75 ± 1.89 | 26.71 ± 1.25 | 29.24 ± 1.85 | 35.77 ± 2.73 | †Pi × PKA × MYBPC3 |
| 8 | 27.63 ± 2.06 | 27.95 ± 1.44 | 31.46 ± 1.81 | 38.87 ± 3.09 | |
| 12 | 29.44 ± 2.23 | 28.49 ± 1.59 | 34.43 ± 2.04 | 42.71 ± 2.97 | |
| Δ2πc/ΔPi | 1.56 ± 0.13 | 1.52 ± 0.16 | 1.48 ± 0.13 | 2.29 ± 0.31 | *PKA × MYBPC3 |
Values are means ± SE; n, number of strips. The sensitivity of 2πc to Pi was calculated and denoted here as Δ2πc/ΔPi. Statistical results reflect repeated-measures ANOVA. Two-way ANOVA was then applied to Δ2πb/ΔPi. *P < 0.05, †P < 0.01. NTGβ, nontransgenic; t/tβ, transgenic lacking cMyBP-C; PKA, protein kinase A.