Figure 4.

Cardiac gene expression in Nebl^ex3-knockout (KO) mice and nebulette (Nebl)-based putative stress network. A and B: Venn diagram and heat-map of differentially expressed genes (DEGs) between sedentary and exercised Nebl^+/+ and Nebl^ex3-/ex3- mice. C: results of principal component analysis (PCA), showing distinguished clustering of exercised mice from the sedentary groups. D: putative cardiac Nebl-based Z-disk regulatory network constructed based on a genetic correlation analysis of cardiac Nebl (black) expression with cardiac transcriptomes across 40 BXD strains and their two parental strains. WT, wild type. Twenty-two Z-disk related genes significantly (P < 0.05) correlated with cardiac Nebl and with each other were identified. Red nodes represent DEGs with a significance between Nebl^ex3-/ex3- sedentary and Nebl^+/+ sedentary. Green nodes represent DEGs with a significance between Nebl^ex3-/ex3- stress and Nebl^ex3-/ex3- sedentary. Negative genetic correlation between genes is indicated by red edges, and green edges indicate positive correlation (P < 0.05).