Figure 3.

Global gene expression. A: principal component analysis demonstrated that experimental groups were well separated by their mRNA expression profiles at postnatal day (P)8 (n = 5 hypoxic, n = 4 control biological replicates), but this separation was negligible in P30 samples (n = 5 hypoxic, n = 4 control biological replicates). B: a total of 1,427 mRNAs were differentially expressed between hypoxic (Hx) and normoxic control (Nx) hearts at P8, with near resolution of differences by P30 [ANOVA with 1.5-fold expression cut-off, P < 0.05 and false-discovery rate (FDR) q ≤ 0.1]. C: specific genes important to cardiovascular functioning and development are highlighted in a volcano plot of all differentially expressed genes between groups at P8.