Contractile function. A: hypoxic animals had decreased contractile function at P30 compared with control, as measured by fractional shortening (FS), fractional area change (FAC), and ejection fraction (EF). B: left ventricular internal diameter (LVID), interventricular septum (IVS), and left ventricular posterior wall (LVPW) were measured in systole and diastole from short-axis M-mode images (i); long-axis traces of the endocardial border were performed in systole and diastole for calculation of EF via a modified Simpson’s monoplane method (ii); and short-axis traces of the endocardial border were performed in systole and diastole for calculation of fractional area change (iii). C: there was no difference between groups in either interventricular septal thickness or left ventricular posterior wall thickness in both systole (IVSs, LVPWs) and diastole (IVSd, LVPWd). Data expressed as means ± SE. *P < 0.05 via two-tailed t test. Biological replicates are shown. ns, not significant.