Figure 9.

Differential gene expression in the aorta after BAPN treatment with and without exercise. Expression level (2^−ΔCT) of selected genes involved in TGFβ pathway (top row), inflammation (middle row), and vascular injury and response (bottom row) related genes in CONV, BAPN-CONV, and BAPN-EX in 10–11-wk-old mice quantified by qRT-PCR. Gene expression is analyzed with one-way ANOVA with post hoc Tukey’s tests for multiple comparisons and is normalized to GAPDH. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. n = 4 biological replicates per group. Extreme outliers, missing data, and unamplified transcripts are excluded. BAPN, β-aminopropionitrile; BAPN-CONV, conventional cage activity; BAPN-EX, forced treadmill exercise.