Figure 3.

High glucose causes increased c-Myc by activating the WNT/β-catenin pathway (A) Total β-catenin expression was analyzed by western blot. (B) The expressions of β-catenin and c-Myc in the cytoplasm and nucleus were analyzed by western blot. (C) The expression of β-catenin was determined by immunofluorescence assay. The nuclei were stained blue using DAPI. Magnification: ×200, Scale bar = 50 μm. (D, E) HaCaT cells were pre-treated with Wnt/β-catenin inhibitor (KYA1797K, 25 μM) for 24 h, and then expressions of β-catenin, c-Myc (D), TGM1, LOR, and K1 (E) were analyzed by western blot. Data were shown as the mean ± SEM measured in triplicate from three independent experiments. ANOVA followed by Tukey’s multiple comparisons test were performed to analyze the differences. **P < 0.01.