Skip to main content
. 2021 May 14;12:676403. doi: 10.3389/fendo.2021.676403

Figure 4.

Figure 4

Inhibition of c-Myc transcription activity alleviates the differentiation dysfunction caused by overexpression of c-Myc or high glucose (A, B) HaCaT cells were pre-treated with c-Myc transcription activity inhibitor (10058-F4, 20 μM) for 24 h, and then expressions of TGM1, LOR, and K1 were analyzed by western blot. (C–E) The wounds in the Ctrl and DM groups were treated with saline, while those in the DM+10058-F4 group were topically treated with 10058-F4 (30 mg/kg) every day for 11 consecutive days. Expressions of TGM1 (C), LOR (D), and K1 (E) were determined by immunofluorescence assay in day 11 wound margin tissues of rats. The nuclei were stained blue using DAPI. Magnification: ×200, Scale bar = 50 μm. The white dotted line represents the epidermis-dermis dividing line. OE-NC, the negative control of c-Myc overexpression. Data were shown as the mean ± SEM measured in triplicate from three independent experiments. ANOVA followed by Tukey’s multiple comparisons test were performed to analyze the differences. *P < 0.05, **P < 0.01.