Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 28;11:11283. doi: 10.1038/s41598-021-90643-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analysis of β-cell proliferation in dispersed human islets by immunocytochemistry for Nkx6.1. (a–g) Dispersed human islets were exposed to 5.5 mM glucose (Vehicle; a–c), 2.8 mM glucose (Vehicle or 2.8 Glu; d–g), harmine (10 μM) (Har; a–c), 16.7 mM glucose (16.7 Glu; d,e,g) and HB-EGF (100 ng/ml) (HB; d,f,g) for 72 h. EdU (10 μM) was added throughout. Proliferation was assessed by EdU staining and Nkx6.1 to mark β cells. Representative images of Nkx6.1 (green), EdU (red) and nuclei (blue) staining are shown (a,d). Arrows and arrowheads highlight EdU⁺/Nkx6.1⁺ and EdU⁺/Nkx6.1⁻ cells, respectively. Scale bar, 100 μm. Images were acquired using an Operetta CLS with Harmony 4.5 software (https://www.perkinelmer.com/en-ca/product/operetta-cls-system-hh16000000). Between 1000 and 1500 Nkx6.1⁺ cells were counted for each sample. Proliferation is presented as the percentage of EdU⁺/Nkx6.1⁺ cells over the total Nkx6.1⁺ cells. Graphs showing cell proliferation of individual (b,e,f) and combined (c,g) donors were generated using GraphPad Prism 9 software (https://www.graphpad.com/scientific-software/prism/). Donor identifiers (Supplementary Table S1 online) are indicated. Significance was tested using paired t tests (b,c,e,f) or one-way ANOVA (g). P < 0.05 was considered significant. NS not significant.