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. 2021 May 28;4:638. doi: 10.1038/s42003-021-02136-8

Fig. 2. Platinum agents induce an upregulation of CDCA3 protein.

Fig. 2

a Representative endogenous CDCA3 western blot analysis from lysates of NSCLC cell lines treated in the absence or presence of cisplatin. Tubulin used as loading control. b Densitometry quantification of a, with dot points representing average log2 of relative CDCA3 levels from three independent experiments with lines connecting respective untreated and cisplatin treated cell lines (paired Student’s t test, *P = 0.0174). c Graphical representation of CDCA3 transcripts from NSCLC cell lines treated with or without cisplatin determined using qRT-PCR. Comparative CT values normalised to housekeeping transcript 7SL and relative to untreated controls for each cell line. Mean ± SD from n = 3 in triplicate. d, e Endogenous CDCA3 western blot analysis of H460 (d) and SKMES-1 (e) lysates assessing protein turnover by treating with cycloheximide over 4 h in the presence or absence of cisplatin. Tubulin used as a loading control. Densitometry quantification of CDCA3 levels relative to 0 h indicated below CDCA3 blots.