Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 28;12:3206. doi: 10.1038/s41467-021-23530-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Schematic for stoichiometry determination of αSNAPs bound to surface-immobilized SNARE-incorporating vesicles. b Representative fluorescence intensity traces for the single spots in the image with the corresponding number of photobleaching step(s). c Representative fluorescence images of Alexa647-labeled αSNAP and DY549-labeled NSF from a total internal reflection fluorescence (TIRF) microscope and the corresponding counts of proteins and co-localized portions. d Deconvoluted histogram of the photobleaching step(s) for labeled αSNAP in single spots examining the number of αSNAP for NSF binding. The histogram of photobleaching step(s) in d was deconvoluted accounting for the 90% labeling efficiency. Error bars in (c, d) represent mean ± s.d. for n = 9 images from two independent experiments (c) and n = 12 images from three independent experiments (d). The reproducibility of the images (b, c) was confirmed in three independent experiments. Source data are provided as a Source Data file.