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. 2021 May 28;12:3241. doi: 10.1038/s41467-021-23461-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Example outputs from HIT-MAP visualisation of bovine lens protein arpin, showing the series of peptide data and peptide ion image for a given parent protein, as well as protein coverage (scale bar = 3 mm). b Visualisation of the integrated clustering of each individual peptide ion image to demonstrate the overall summary spatial distribution of an annotated protein. Intensity scales represent relative intensity from 0 to 100%. Scale bar = 3 mm.