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. 2021 May 28;12:3227. doi: 10.1038/s41467-021-23386-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a–c Time-lapse images of the CAG::H2B-EGFP nuclear reporter (gray) in E13.5–E14.5 skin-roll explants taken every 7 min. a, b Examples of perpendicular dividing basal cells with daughter cells that point away from the epidermal-dermal interface that was either integrated into the basal layer after division ((a), 44 out of 94 cells), or remained in the second layer for the duration of the analysis ((b), 50 out of 94 cells). c An Example of a delaminating cell that left the basal layer moved suprabasally and divided (a total of 46 cells observed). Note the other dividing suprabasal cell (red asterisks) (scale bars: 10 µm). The data were obtained from five explants taken from five different embryos in five independent imaging experiments. The dashed line represents the epidermal-dermal interface in all panels. Yellow dots mark the cells of interest. d Immunostaining of back-skin sections at the indicated stages stained for ITGA6 (red) and K10 (green) (scale bar: 25 µm). Arrowheads indicate cells in the second layer that are positive for ITGA6 but negative for K10.