Fig. 3. Specificity of AURKA targeting by PROTAC-D.

a U2OS cells expressing tet-regulated AURKA-Venus and AURKB-Venus were arrested in mitosis with STLC and treated with 250 nM PROTAC-D for 3 h. Scatter plots with mean values and SDs indicated show percentage degradation of protein in individual cells pooled from three separate experiments, statistical test applied was Mann–Whitney U-test. b–d Endogenous levels of AURKA interactors TPX2 and TACC3, or of AURKB, were examined by quantitative immunoblotting of extracts from cells arrested in mitosis with STLC and treated with PROTAC-D or vehicle control (DMSO). b HeLa cells treated for 3 h, immunoblot representative of data from three identical experiments quantified and presented in c, showing mean percentages of protein remaining after PROTAC-D treatment, relative to DMSO treatment, with error bars to indicate SDs. d RPE1 AURKA-Venus^KI cells treated for 12 h, immunoblot from one experiment.