Fig. 7. PROTAC-D treatment restores the mitochondrial network in FZR1^KO cells.

U2OS FZR1^KO cells, in which mitochondria are highly fragmented due to the presence of excess AURKA²⁴, were treated for 3 h with DMSO or with MLN8237, Cpd A or PROTAC-D at the doses indicated. Cells were then stained with MitoTracker Red^TM, imaged and analysed for mitochondrial fragment lengths as described in materials and methods. a Representative images of cells analysed. Scalebar, 10 μm. b Scatter plots showing mean mitochondrial lengths, mean and SDs under different drug treatments. Each datapoint represents the mean value from 30 mitochondria per cell, n ≥ 12 cells, with results tested for significance using Mann–Whitney U-test. The data are from a single experiment that is representative of two identical repeats of the same experiment.