Fig. 6. Identifying aminformatide (AMINF) NRP family discovered by NRPminer in the SoilActi dataset.

a The BGC generating the core NRP in Amycolatopsis sp. AA4 along with NRPS genes (shown in red) and the A-, C-, PCP, and E-domains appearing in the corresponding NRPS. The rest of the genes in the corresponding contigs are shown in white. Three highest-scoring amino acids for each A-domain in this BGC (according to NRPSpredictor2 (ref. ¹⁵) predictions) are shown below the corresponding A-domains. Amino acids appearing in the NRP VVIVETRY identified by NRPminer (with the lowest p value) are shown in blue. b Spectral network formed by spectra that originate from the AMINF NRPs. A node is colored if the corresponding spectrum forms a statistically significant PSM and not colored otherwise. The p values are computed based on MCMC approach using MS-DPR⁸⁹ with 10,000 simulations. c Sequences of the NRPs identified by NRPminer in the aminformatide family (with the lowest p value among all PSMs originating from the same NRP). NRPminer predicted a PAM with loss of ~0.96 Da on E, represented by E*. AMINF represents aminformatide. d For each identified NRP, an annotated spectrum representing the lowest p value is shown.