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. 2021 May 28;12:3221. doi: 10.1038/s41467-021-23477-2

Fig. 7. The Theileria TaSETup1 can methylate H3K18 and repress gene expression.

Fig. 7

a Methyltransferase assay of recombinant TaSETup1 (wild-type WT or mutant H206F) incubated with or without SAM and core histones followed by immuno-dot-blot detection with H3K18me1 antibody. The quantification results were normalized to H3. This result is representative of three independent experiments showing similar outcomes. b Assay of TaSETup1 methyltransferase activity by quantification of fluorescent H3K18 peptide (substrate S and methylated product P). Chromatograms show mono-methylated (K18me1) and di/tri methylated (K18me2/3) product in presence or absence of SAM for WT and mutant enzymes. Inset: Integration of the peak (AUC area under the curve). c Recruitment of GAL4-TaSetup1 (but not the GAL-TaSETip1 H206F mutant) caused repression of the Luciferase transgene in T-Rex cells in the presence Tetracycline (48 h). The % Luciferase activity represents the mean of n = 3 biologically independent experiments. Error bars represents mean values ± SD.