Fig. 4.

Inhibition of lysosomal Ca²⁺ release through TRPML1 channel by Hcy. A. Representative images showing GCaMP3 fluorescence in different groups of podocytes. Scale bars = 40 μm. B. A representative curve showing that ML-SA1 induced remarkable elevation of GCaMP3 signal in podocytes under control condition. C. A representative curve showing that ML-SA1 induced small elevation of GCaMP3 signal in podocytes after Hcy stimulation. D. Summarized data showing GCaMP3 fluorescence in different groups of podocytes (n = 5–9). *p < 0.05 vs. Vehl group, #p < 0.05 vs. Ctrl group. Ctrl, control; Vehl, vehicle; gp91 ds-tat, gp91 ds-tat peptide; Hcy, homocysteine.