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. 2021 Apr 28;51:101239. doi: 10.1016/j.molmet.2021.101239

Figure 2.

Figure 2

Bioenergetic consequences of MICU2 knockdown in INS-1 832/13 cells. Glucose-induced mitochondrial hyperpolarization of the inner mitochondrial membrane upon MICU2 knockdown in INS-1 832/13 cells measured by TMRM (A and B); average traces (A) and difference in maximal hyperpolarization (B) between control and MICU2-silenced cells are shown. The cytosolic ATP/ADP ratio in response to an elevation in glucose from 2.8 to 16.7 mM upon MICU2 knockdown in single INS-1 832/13 cells monitored with Perceval HR (C and D); average traces (C) and difference in the maximal increase in the ATP/ADP ratio (D) between control and MICU2-silenced cells are shown. Data are from at least three independent experiments 72 h after adding siRNA. Mean ± SEM are given. Comparisons were made with an unpaired two-tailed Student's t-test. ∗∗p < 0.01 and ∗∗∗∗p < 0.0001.