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. 2021 Apr 23;296:100700. doi: 10.1016/j.jbc.2021.100700

Figure 2.

Figure 2

ΔyhcB lacks proper stationary state growth regulation.A, the ΔyhcB and its parental strain BW25113 (WT) were grown in LB at 37 °C for 24 h (overnight [ON]) and then diluted 1:1000 and grown to an absorbance at 650 nm of 0.3 (EXP[onential]) or to an absorbance at 650 nm of 1.2 (STAT[ionary]) and fixed, and the nucleoids were stained with 4',6-diamidino-2-phenylindole (DAPI). The length, diameter, and DAPI fluorescence of each culture are indicated with the mean and SD. The number of analyzed WT cells was 434 (EXP), 1225 (STAT), and 2133 (ON), respectively. The number of analyzed ΔyhcB cells was 555 (EXP), 776 (STAT), and 811 (ON), respectively. B, representative images form all six cultures with WT on the left and ΔyhcB on the right. The images are merged phase-contrast (gray) and DAPI (green) images with a blue background for optimal contrast. Brightness, contrast, and scale are the same for all images.