Figure 1.

Characterization and validation of two-layer microfluidic device for thermal stimulation of Hydra vulgaris

(A) (Left) Photograph of microfluidic device with blue dye in flow layer and green dye in Hydra chamber. Scale bar, 1 mm. (Middle) Schematic of cross-section of the device. (Right) Legend for left and middle panels.

(B) Infrared image showing temperature of the flow layer before (leftmost), the start of (second leftmost), during (center), end of (second rightmost), and after (rightmost) thermal stimulation.

(C) Time course of thermal stimulation at temperatures above and below Hydra's baseline culture temperature of 18°C.