Figure 6.

Y311 on PKCδ regulates thromboxane generation.A, representative western blots of platelet proteins from WT and Y311F mice. Platelets were stimulated with the indicated concentration of AYPGKF, and the membranes were probed for phosphorylated ERK1/2 and total ERK1/2, which was used to assess loading. B, quantification from multiple experiments described in B, expressed as a ratio of phosphorylated ERK1/2 to total ERK1/2. C, washed platelets from WT control and PKCδ Y311F mice were stimulated with the indicated concentration of AYPGKF for 3 min under stirring conditions. Thromboxane generation was assessed using a kit from Enzo Scientific. ∗p < 0.05, n = 5. ERK1/2, extracellular signal-regulated protein kinase; PKC, protein kinase C.