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. 2021 Apr 30;296:100728. doi: 10.1016/j.jbc.2021.100728

Table 1.

Results of the SMFS experiments performed under different buffer conditions

Buffer xβ [Å] koff [s−1] n (FDCs) n (datasets)
PBS 2.85 ± 0.043 3.61 ± 0.015 13,106 7
pH 5 (NaAc) 2.42 ± 0.043 4.44 ± 0.020 13,967 9
H2O 2.96 ± 0.039 3.43 ± 0.014 21,096 8

Width of the energy barrier (xβ) and kinetic off-rate (koff) were calculated from the FDCs according to Evans theory using a most likelihood approach.