Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 29;11:100. doi: 10.1186/s13578-021-00609-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — RDS dosage-dependent inactivation of the infectivity of SARS-CoV-2 and derived Ha-CoV-2 variants. A SARS-CoV-2 particles were incubated with serially diluted RDS at 37 °C for 1 h. Subsequently, the mixture were further serially diluted and added to Vero cells for plaque assays to determine reduction of virus infectivity. Inhibition assays were performed in triplicate, and statistical significance was determined using One-Way ANOVA with Dunnett’s Post Test in Prism 7 (Graph Pad). Significance values are indicated using asterisks as follows; *p < 0.02. The designation "ns" stands for "not significant". B Ha-CoV-2(Luc) and related S protein variants were incubated with serially diluted RDS at 37 °C for 1 h. Subsequently, the mixture were used to infect HEK293T (ACE2/TMPRESS2) target cells. Inhibition of viral infection was quantified at 12 h post infection by luciferase assay. The IC₅₀ values of RDS were calculated to be at dilutions of 1:177 (wt), 1:828 (B.1.1.7), 1:124 (B.1.351), 1:88 (P.1), 1:134 (B.1.1.207), 1:2601 (B.1.1.298), 1:70 (B.1.258), 1:362 (B.1.429), 1:163 (B.1.494), 1:137 (B.1.2)