hPSC growth medium, store at 4°C for 3 months

Reagent	Final concentration	Amount
DMEM/F-12	N/A	500 mL
KnockOut Serum Replacement	20%	127.4 mL
GlutaMAX	0.5%	3.2 mL
Nonessential amino acids (NEAA)	1%	6.4 mL
bFGF (add before use)	4 ng/mL
Total	N/A	637 mL

Filter through a 0.22 μm vacuum driven filter.