2×Transfection Buffer, store at 4°C for 3 months

Reagent	Final concentration (for 2×transfection buffer)	Amount
HEPES	0.01 g/mL	5 g
NaCl	0.016 g/mL	8 g
Na2HPO4	0.0002164 g/mL	0.1082 g
KCl	0.00076 g/mL	0.38 g
Glucose	0.002 g/mL	1 g
ddH2O	N/A
Total	N/A	500 mL

pH 7.04 ~7.05, adjusted with 3 M NaOH. Filter through a 0.22 μm vacuum driven filter.