MEF growth medium, store at 4°C for 3 months

Reagent	Final concentration	Amount
DMEM (1×)	N/A	500 mL
Fetal bovine serum (FBS)	10%	56.2 mL
Nonessential amino acids (NEAA)	1%	5.6 mL
Total	N/A	561.8 mL