| Component | Amount per reaction (μL) |
|---|---|
| 5×Reaction Buffer | 10 |
| 10 mM dNTPs | 1 |
| sgRNA-PCR-F | 1 |
| sgRNA-PCR-R | 1 |
| sgRNA library | 100 ng in 10 μL |
| PrimeSTAR-GXL polymerase | 1 |
| ddH2O | 26 |
| Total | 50 |
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