| PCR cycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Initial denaturation | 98°C | 60 s | 1 |
| Denaturation | 98°C | 15 s | 20 |
| Annealing | 55°C | 15 s | |
| Extension | 68°C | 20 s | |
| Final extension | 68°C | 5 min | 1 |
| Hold | 4°C | Forever | 1 |
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