Table 1.

Flow cytometry antibodies and reagents to measure CD8+ purity after T cell isolation per 100,000 T cells

Stain	Stain dilution
SYTOX Blue Viability Dye	1 in 1,000 in FACS Buffer
CD3 in PE	1 in 100 in FACS Buffer
CD4 in FITC	1 in 100 in FACS Buffer
CD8 in APC	1 in 100 in FACS Buffer