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Flow cytometry antibodies and reagents to measure extracellular expression of activation markers on CD8+ T cells
| Stain | Stain dilution |
|---|---|
| Viability Dye Fixable Aqua | 1 in 2,000 in PBS |
| CD8 or CD3 in FITC | 1 in 100 in FACS Buffer |
| CD25 PE | 1 in 100 in FACS Buffer |
| CD69 APC-Cy7 | 1 in 100 in FACS Buffer |
| HLA-DR APC | 1 in 100 in FACS Buffer |
