T cell culture medium with IL-2
Reagent	Final concentration	Amount
RPMI 1640 media	n/a	440 mL
L-glutamine, Penicillin streptomycin (100×)	1×	5 mL
HEPES (1 M)	10 mM	5 mL
Human AB serum	10%	50 mL
IL-2 (resuspended stock)	10 IU/mL	5000 IU in 10 μL
Total	n/a	500 mL

Store at 4°C for up to 1 month.