Figure 3. Combination of orlistat with enzalutamide enhances the reduction of related proteins by inhibiting the phosphorylation of AKT and ERK proteins.

The cell culture conditions and drug treatments were as described in the legend of Figure 2. The results showed that orlistat, enzalutamide and combination treatments all reduced the expression of FASN, MMP-9, NF-κB, p-AKT, VEGF, p-ERK, cyclin D1 and Bcl-2 in PC3 cells. Both orlistat and enzalutamide suppressed protein expression in a dose-dependent manner. All experiments were repeated at least three times for the calculation of alteration in fold, while only one repeat was randomly selected for presentation. Abbreviation: p, phosphorylated.