Fig. 2.

A Scatterplot shows ABCG2 stands out as the single gene showing a highly significant change in expression with use of the ABCG2_A and ABCG2_B sgRNAs to activate ABCG2 expression. These data are generated from RNA-sequencing of MiaPaca-2 and Panc-1 cells over-expressing ABCG2. B Cell survival curves display the fraction of cells surviving after a series of irinotecan doses for Panc-1 cells stably expressing an ABCG2-targeting sgRNA (blue) or non-targeting control sgRNAs (red and black). C Each bar represents a ratio of ABCG2 over-expressing cells compared to a non-targeting control with the indicated drug treatments (+ indicates a treatment was added). Treatment of MiaPaca2 cells over-expressing ABCG2 using the ABCG2 guide shows no significant cell death with inhibitor only, where as ABCG2 alone confers drug resistance. The combination of 3uM sorafenib (green) or KO143 (maroon) with ABCG2 overexpression restores normal sensitivity to Irinotecan. D Boxplots showing the normalized expression levels of ABCG2 in Kirby et al. for patients surviving < 300 days or > 900 days. P-value is not significant