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. 2021 May 26;62(6):26. doi: 10.1167/iovs.62.6.26

Figure 4.

Figure 4.

Baicalein inhibits the expression of proinflammatory mediators induced by A. fumigatus in vivo and in vitro. In mouse model of keratitis, RT-PCR showed the mRNA levels of IL-1β (A), IL-6 (C), and TNF-α (E) were reduced significantly in baicalein (concentration = 0.25 mM) vs. DMSO-treated corneas at one, three, and five days p.i. ELISA analysis for protein levels of IL-1β (B), IL-6 (D), and TNF-α (F) at three and five days p.i. in baicalein- versus DMSO-treated mice (n = 6/group). In HCECs, RT-PCR showed the mRNA levels of IL-1β (G), IL-6 (I) and TNF-α (K) were reduced significantly in baicalein (concentration = 0.25 mM) vs. DMSO-treated cells at four hours after infection. ELISA analysis for protein levels of IL-1β (H), IL- 6 (J), and TNF-α (L) at 24 hours following infection in baicalein- versus DMSO-treated HCECs. All data are mean ± SEM and were analyzed using an unpaired, two-tailed Student's t-test (ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001).