Figure 6.

Baicalein inhibited A. fumigatus–induced expression of TSLP in HCECs. RT-PCR demonstrated mRNA expression level of TSLP in HCECs treated with 0.25 mM baicalein or DMSO at four hours after A. fumigatus infection (A). Western blotting showed TSLP protein expression level in baicalein (0.25 mM) or DMSO-treated HCECs at 24 hours after infection (B) and quantitative analysis (C). Immunofluorescence images of TSLP (green) expression in A. fumigatus–infected HCECs with or without 0.25 mM baicalein treatment at eight hours after infection (D) and quantitative analysis (E). Green: TSLP (FITC), blue: nucleus (DAPI), magnification 200×. All data were mean ± SEM and were analyzed by an unpaired, two-tailed Student's t-test (ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001).