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. Author manuscript; available in PMC: 2021 May 29.
Published in final edited form as: Cell Rep. 2021 May 4;35(5):109080. doi: 10.1016/j.celrep.2021.109080

Figure 4. Microglial process pouches do not show phagocytic lysosomal activity.

Figure 4.

(A–C) Representative confocal image of a fixed slice stained with DAPI, CD68, CX3CR1-GFP, and LysoTracker.

(D and E) Representative two-photon in vivo images from CX3CR1GFP/+ mice after KA treatment and treated with LysoTracker. Arrows indicate pouches, and arrowheads indicate LysoTracker label. Scale bar: 10 μm.

(F) Quantification of the number of pouches of various sizes observed.

(G) Quantification of the number of LysoTracker+ pouches of various sizes observed. Data are presented as mean ± SEM.