Figure 2.
Effects of curcumin on cell cycle, DNA damage response, and cell death. PC-3 and PC-3AcT cells were treated with the indicated concentrations of CCM in DMEM containing lactic acid (3.8 μM) for 48 h. (a) Cell cycle distribution was determined by flow cytometry following PI (20 μg/mL) staining. (b) The levels of DNA damage response proteins were assessed by Western blotting. (c) Cell death fraction was analyzed using annexin V-PE binding assay. ∗P < 0.05 vs. respective control cells. #P < 0.05 vs. respective PC-3 cells. CCM: curcumin. Arrows, sub-G0/G1 peak.