Extended Data Fig. 11 |. Infusion of 5-HT1b receptor agonist into the NAc increases sociability, but does not alter control behaviours.

a, Schematic of 5-HT1b receptor agonist (CP93129) infusion into the NAc in Sert-cre or Sert-cre:16p11.2^flx mice. b, Timeline of behavioural experiments. c, d, Quantification of chamber and proximity time in the three-chamber assay (c: vehicle, F_2,15 = 45.82, P < 0.001 (left); F_3,30 = 4.453, P < 0.05 (right), n = 6; d: CP93129, F_2,15 = 63.11, P < 0.001 (left); F_1,10 = 17.23, P < 0.05, (right), n = 6) in Sert-cre mice with either vehicle (red) or CP93129 (aqua) infused into the NAc before analysis of behaviour. e, f, Quantification of chamber and proximity time in the three-chamber assay (e: vehicle, F_2,12 = 67.49, P < 0.001(left); F_1,8 = 41.03, P < 0.001 (right), n = 5; f: CP93129, F_2,12 = 29.73, P < 0.001 (left); F_1,8 = 17.17, P < 0.01 (right), n = 5) in Sert-cre:16p11.2^flx mice with either vehicle (blue) or CP93129 (pink) infused into the NAc before analysis of behaviour. g–i, Quantification of the novel object interaction assay (g: F_1,18 = 1.612, P = 0.2204, n = 5–6), locomotion assay (h: F_1,18 = 0.0149, P = 0.9041, n = 5–6), and centre time (i: F_5,50 = 1.492, P = 0.2093; F_5,40 = 0.4561, P = 0.8063, n = 5–6) in Sert-cre and Sert-cre:16p11.2^flx mice with either vehicle or NAS-181 infused into the NAc before behaviour. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; repeated measures, two-way ANOVA with Tukey’s multiple comparison post hoc test. Comparisons with no asterisk had P > 0.05 and were considered not significant. The schematic of the mouse brain in this figure has been adapted with permission from Franklin & Paxinos⁴⁶.