Fig. 2.

PLAC8 regulates MCF-7/TAM and MCF-7 cells proliferation. a The protein (upper) and mRNA levels (bottom) were relatively higher in MCF-7/TAM cells than in MCF-7 wild type. b MCF-7/TAM was transfected with PLAC8 siRNA; the interference effect of siRNA was determined by Western blot (upper) at 72 h after transfection and RT-PCR (bottom) at 48 h after transfection. c and d MTT assay was used to determine the cell viability of PLAC8 overexpressing cells or siRNA transfected cells. Control and Si-NC were chosen as the control. e and f Equal amount of MCF-7 cells or MCF-7/TAM cells under different PLAC8 condition was cultured with the various concentrations of tamoxifen for 48 h. Cell viability was determined by MTT assay.All data represent mean ± SD of three experiments performed in triplicate. **p < 0.01; *p < 0.05; NS, not significant