Fig. 2.

Myostatin induces Fgf23 expression in UMR106 cells, a Arithmetic means ± SEM of relative (rel.) Fgf23 mRNA abundance or b C-terminal or c intact FGF23 protein concentration in the cell culture supernatant of UMR106 osteoblast–like cells incubated without (white bars) or with (black bars) myostatin (a indicated concentrations, n = 7; and b, c 100 ng/mL, n = 10) for 24 h. *p < 0.05, **p < 0.01, ***p < 0.001 indicate significant difference from control. AU, arbitrary units; ctr, control (a one-way ANOVA; b unpaired Student’s t test with Welch’s correction; c Mann–Whitney-U-test)