Fig. 3.

Hemogenic fate specification and HSC emergence from hemogenic endothelium (HE). a Schematic cross-section through the murine E10.5 aorta–gonad–mesonephros (AGM) region. The dorsal aorta (DA) is boxed (red dashed box) and magnified to visualize the cell types involved in AGM hematopoiesis. Hematopoietic cells, including pre-HSCs, arise through an intermediate hemogenic endothelium (green), organized in intra-aortic hematopoietic clusters (IAHCs). This endothelial-to-hematopoietic transition (EHT) and HSC emergence is regulated and directly or indirectly influenced by signaling and cell-extrinsic factors from the microenvironment (e.g., vascular endothelial cells (light blue) and perivascular mesenchyme (purple))