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. 2021 Mar 19;147(7):1937–1955. doi: 10.1007/s00432-021-03597-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Presentation of relative mRNA expression of glypican genes within a specific cell line. Gene expression of GPC1, GPC2, GPC3, GPC4, GPC5 and GPC6 was quantified by qPCR in 7 breast cancer cell lines representative of the luminal (MCF-7 and T47D), basal (MDA-MB-231, -453 and -468) and HER2-positive (BT474 and SKBR3) breast cancer subtypes. Gene expression was normalized to the expression of β-actin and is represented by 2^−ΔCt. Error bars indicate the mean and standard deviation (SD) from three individual experiments conducted in triplicates