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. 2021 May 29;7:126. doi: 10.1038/s41420-021-00508-x

Fig. 4. ETV4 promotes breast cancer cell stemness by activating the Sonic HH signaling.

Fig. 4

A GSEA plot showing significant enrichment of hedgehog signaling in TCGA breast cancer tissues with high ETV4 expression. B, C Immunoblots of SHH and GLI1 in monolayer MCF7 cells and MCF7-derived spheres (B) and in MDA-MB-231 and MCF7 cells with or without ETV4 depletion (C). D Representative confocal images for GLI1 (green) in MDA-MB-231 cells with or without ETV4 knockdown. Scale bar =10 μm. E Immunoblots of SHH and GLI1 in the indicated breast cancer cells with or without ETV4 overexpression. F, G Representative images of spheres formed by ETV4-overexpressing MCF7 cells treated with or without 10 μM Vismodegib (F) and boxplots or histograms showing the diameters and numbers of spheres (G). A box represents the distribution of sphere diameter and a upper (lower) whisker represents the max (min) diameter. Data are presented as mean ± SD, n = 3. *P < 0.05, **P < 0.01.