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. 2021 May 29;7:126. doi: 10.1038/s41420-021-00508-x

Fig. 6. ETV4 loss retarded growth of breast tumor xenograft.

Fig. 6

A Efficient transduction of MDA-MB-231 cells by GFP-expressing lentiviral vector carrying short shRNAs for ETV4 as reflected by green fluorescence. B, C Establishment of stable ETV4 knockdown MDA-MB-231 cells as confirmed by quantitative RT‐PCR (B) and western blotting assay (C). D–F Nude mice (n = 6 each group) were subcutaneously implanted with control or shETV4 MDA-MB-231 cells. Tumor volumes were measured (D). Twenty-five days after inoculation, tumors were isolated (E) and weighted (F). G Immunoblots of ETV4, CXCR4, SHH and GLI1 in harvested MDA-MB-231 tumors. Data are presented as mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.